Lab-on-a-Chip System for Detection of Mycotoxins in Animal Feed
Poster Apr 04, 2012
S. Brunklaus, V. Stein, M. Jakubowski, M. Ritzi-Lehnert, K.S. Drese, R. Colasanto, P. Poletti
Mycotoxins are health hazardous metabolites produced by fungi growing on foodstuffs or animal feed. Upon exposure they impact on the productivity of livestock including diary products. As the result of livestock feeding on contaminated commodities, mycotoxins carry over to the human food chain via meat or other animal products. This poses a high risk to human health as some of the most common mycotoxins are carcinogenic, genotoxic, or target the kidney or liver. It is now well established that acute mycotoxicoses (the diseases caused by mycotoxins) have been responsible for major epidemics in man and animals. Since mycotoxins are robust, small molecules that are not easily destroyed by temperature treatments occurring during cooking, baking or freezing processes and even fermentation, it is necessary to avoid usage of contaminated food and feed upfront. However, the presence of mycotoxins is not detectable by visual screening due to the fact that high amounts of toxins may still exist even after removal or destruction of the generating organisms. Hence availability of sensitive, accurate and affordable tests for the detection and quantisation of mycotoxin in feeds is of great interest for the care of health.
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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE