LC-MS/MS METHOD FOR THE QUANTIFICATION OF PITOFENONE IN HUMAN PLASMA
Poster Nov 25, 2014
B.M. Gurupadayya, S. Sharfuddin and R.S. Chandan
A simple, reverse-phase high performance liquid chromatographic method with mass spectrometric detection (HPLC-MS/MS) was developed for determination of Pitofenone in human plasma using Fenpiverinium Bromide as an internal standard. The procedure involves a simple protein precipitation technique using ZORBAX ECLIPSE XDB – C-18, 4.6 × 150 mm, 5µm, column. The mobile phase used was methanol: 10 mM ammonium acetate in (70: 30). Precipitation was done using methanol and detection was done in MRM mode, using an Electro Spray positive ionization. The ion transition monitored was (m/z) Pitofenone (Q1 Mass: 368.1;Q3 Mass 112.1), Internal standard (Q1 Mass: 338.3;Q3 Mass 239.1). The retention time of Pitofenone and Internal Standard were 3.2 and 1.7 respectively. Method was evaluated in terms of linearity, accuracy, precision, recovery, sensitivity. The simple extraction procedure and short chromatographic runtime make the method suitable for therapeutic drug monitoring studies.
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The Use of Pyrolysis/GCMS and Newly Developed Libraries to assist in Characterizing Complex Polymeric SamplesPoster
Pyrolysis-GC/MS is an effective tool in introducing and analyzing polymers. But since the polymer must be broken down to volatile segments in order to be sent to the GC, identification by the Mass Spec does not look at the original polymer, but only the fragments. This poster will look at a unique library designed to work with the existing NIST library, however contains averaged spectra of pyrolyzed polymers so it can be used to directly identify the original polymer sample.READ MORE