Low Volume Liquid Handling of Organic Solvents for Compound Storage and Dissolution using mosquito
Poster Feb 28, 2008
Gillian Lewis, Tristan Cope, Joby Jenkins, David Gledhill & Rob Lewis
The technology of storing dried microquots is an interesting new idea which aims to reduce very significantly the amount of material – and in particular of intermediate material – wasted in the process of synthesising new compounds. This technology may also benefit drug screening where there is a similar need to conserve material and where there are also concerns of material stability.
mosquito® (TTP LabTech) is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range.
Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP InhibitorsPoster
Can we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?READ MORE
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE
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NanoBRET offers improved S:B over other BRET assays.
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