Low Volume Liquid Handling of Organic Solvents for Compound Storage and Dissolution using mosquito
Poster Feb 28, 2008
Gillian Lewis, Tristan Cope, Joby Jenkins, David Gledhill & Rob Lewis
The technology of storing dried microquots is an interesting new idea which aims to reduce very significantly the amount of material – and in particular of intermediate material – wasted in the process of synthesising new compounds. This technology may also benefit drug screening where there is a similar need to conserve material and where there are also concerns of material stability.
mosquito® (TTP LabTech) is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range.
Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE