Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
Poster May 11, 2010
Cathy Olsen, Iris Yang, and James Wasson
Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells. Adjustable dispense rate and aspiration height allow users to minimize disruption of even weakly adherent cells. We demonstrate >95% retention of weakly adherent HEK293 cells on a standard tissue culture microplate surface after washing with the AquaMax microplate washer and cell wash head. We also show excellent performance of washed CHO-M1 cells in a Fluo-4 calcium flux assay run on the FlexStation® 3 multimode microplate reader.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE