Metabolite Profiling Using Human Hepatocyte Co-cultures and UHPLC-QTOF-MS with Data Independent MS/MS
Poster Sep 08, 2014
Ronghua Wang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
The identification of human derived metabolites is an imperative step in drug discovery and development process. Typical studies are conducted in human in vitro systems and metabolites are identified utilizing HPLC-MS/MS. Human
hepatocyte co-culture is a new in vitro system, which is comprised of a mixture of hepatocytes and fibroblast cells, capable of remaining viable and highly functional
for up to 4 weeks. In this presentation, the application of high resolution mass spectrometry (HRMS), ultrahigh-pressure liquid chromatography (UHPLC), full scan acquisition, and Sequential Window acquisition of All THeoretical fragment ion spectra (SWATH™) was used to profile and characterize metabolites of
diclofenac, linezolid, and ziprasidone following incubations in human hepatocyte co-cultures.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.