Metabolite Profiling Using Human Hepatocyte Co-cultures and UHPLC-QTOF-MS with Data Independent MS/MS
Poster Sep 08, 2014
Ronghua Wang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
The identification of human derived metabolites is an imperative step in drug discovery and development process. Typical studies are conducted in human in vitro systems and metabolites are identified utilizing HPLC-MS/MS. Human
hepatocyte co-culture is a new in vitro system, which is comprised of a mixture of hepatocytes and fibroblast cells, capable of remaining viable and highly functional
for up to 4 weeks. In this presentation, the application of high resolution mass spectrometry (HRMS), ultrahigh-pressure liquid chromatography (UHPLC), full scan acquisition, and Sequential Window acquisition of All THeoretical fragment ion spectra (SWATH™) was used to profile and characterize metabolites of
diclofenac, linezolid, and ziprasidone following incubations in human hepatocyte co-cultures.
C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitroPoster
Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE
Fingerprinting the Terpene Profiles of Various Cannabis Strains using GC and GCxGC with High Performance TOFMSPoster
The Pegasus BT 4D facilitates fast and confident cannabis product “fingerprinting” through enhanced two-dimensional chromatographic resolution and high performance TOFMS.
Robust compound identification was achieved through spectral similarity searches of large, well-established databases, mass D determinations, and retention index filtering.
High Speed Analysis: Combining Fast GC with Time-of-Flight Mass Spectrometry for Complex Sample Analysis in Under One MinutePoster
The demand for more efficiency and productivity in the laboratory has led to applications using Fast GC-MS methods for routine analysis in a wide range of fields.READ MORE
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9th International Conference on Mass Spectrometry and Chromatography
Sep 21 - Sep 22, 2018