Metabonomics, Dietary Influences and Cultural Differences: A 1H NMR-Based Study of Urine Samples Obtained From Healthy British and Swedish Subjects
Poster Dec 05, 2005
E.M. Lenz, J. Bright, I.D. Wilson1, A. Hughes, J. Morrisson, H. Lindberg and A. Lockton
Metabonomics is well established as a means of disease and toxicity screening in experimental animals.
Metabonomics will find increased application in the study of healthy and diseased humans. However,one of the major obstacles in clinical investigations is the greater variability in a human population.
Here, we describe two investigations on healthy subjects designed to evaluate the variability in metabonomic data, in view of dietary influences and cultural trends.
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE
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