Micro array of Ag-AgCl electrodes for cellular stimulation and sensing
Poster Sep 17, 2014
O. Pilloni*, M. A. Flores*, C. A. Santos*, A. Torres**, J. García**, H. González**, L. Oropeza-Ramos*.
Most novel efforts of miniaturizing electrophysiology techniques consist of designs based on gold and platinum microelectrodes for sensing and stimulation[1,2], which results expensive. In this study, silver–silver chloride as microelectrode material is investigated as an alternative, considering the benefits of its low cost, biocompatibility and high conductivity.
A methodology to design planar microelectrodes for sensing and stimulation of excitable cells is presented, based on the Helmholtz model to analyze the electrode-electrolyte interface impedance and the Arrhenius model to obtain the biological medium electrical properties. Two different electrode geometries are proposed to study both rounded portions and strips of tissue.
The fabrication was performed using lift-off to pattern silver traces on glass substrate, followed by electroplating to grow the silver film and finally coating the electrodes with silver chloride by soaking the traces on iron chloride. An open microfluidic PDMS layer is placed on top to contain the biological medium that keeps the tissue alive during experiments.
The platform was tested using bull frog sciatic nerve, zebra fish and bull frog heart, as samples of excitable cells, giving similar results to conventional macro electrodes. Confirming that Ag-AgCl is a feasible material for microelectrodes aim to sense and stimulate excitable cells.
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAsPoster
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
Designing a Model to Explore Tau's Unfolded Protein ResponsePoster
The purpose of this research is to design a cell model in which ER stress caused by tau accumulation can be generated, and then investigated for changes in different ER stress-associated proteins.READ MORE
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Comments | 0 ADD COMMENT
World Congress on Advanced Structural and Molecular Biology 2018
Aug 22 - Aug 23, 2018