Microfluidic Extrusion of Cell-laden Hydrogel Fibers for 3D-Bioprinting
Poster Oct 13, 2016
Cristina Colosi, Laura Belloni, Giancarlo Ruocco
3D Bio-Printing arises from the intersection between 3D-printing and cell encapsulation techniques, allowing for the creation of bio-constructs with programmable spatial disposition of cellular and extracellular matrix components (bio-ink) . At the Italian Institute of Technology we are developing a bioprinter based on the microfluidic wet-spinning of cell-laden fibers suitable for many different bio-inks. The use of microfluidic platforms coupled with 3D deposition offers the possibility to define the composition and disposition of different bioinks using a single print-head, allowing for the easy creation of heterogeneous constructs.
The microfluidic platforms can be used to vary on-the-fly the density of cell seeding during the printing process, as well as the composition of the embedding ECM. Furthermore, the manipulation of different bioinks at the microscale permits to produce heterogeneous fibers containing different types of cells, disposed in pre-determined patterns, allowing for the creation of complex and highly reproducible co-culture models.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE