MicroRNA Expression Signature in Human Glioblastoma Multiforme Brain Tumor
Poster Sep 22, 2005
Dana Ridzon, Ruoying Tan, Julie Nguyen, Adam Broomer, Caifu Chen
Expression of 180 human miRNAs was examined using recently developed stem loop primers for reverse transcription (RT) followed by real-time PCR. MicroRNAs can be quantified from as few as single cells or as little as 25 pg total RNA. The CT values correlated to the copy number over up to seven orders of magnitude. TheTaqMan® miRNA assays discriminated between two miRNAs that differed by as little as a single nucleotide, and between mature miRNAs and their precursors.
This method allows accurate and sensitive miRNA expression profiling and uncovers precise changes of miRNA expression. Comparing to normal human brain, the glioblastoma multiforme (GBM) tumors have a distinct expression signature of miRNAs. Nearly half of miRNAs showed the reduced expression by > 2-folds. In contrast, only 13% miRNAs had increased expression (>2-folds) in GBM. Expression of miR-10a and miR-10b etc. located within class I HOX and miR-129, miR-139,and miR-153 etc. within class II HOX gene clusters is either elevated or reduced (>10-fold), suggesting that these miRNAs may be involved in brain cancers.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
International Conference on Neurooncology and Neurosurgery
Sep 17 - Sep 18, 2018