MicroRNA Profiling of Breast Cancer using miRCURY™ LNA Arrays
Poster Jan 18, 2007
Thomas Litman, Mikkel Nørholm, Christian Glue, Nina Stahlberg, Nana Jacobsen, Jens Eriksen, Inge M Svane, Henrik Flyger, Eva Balslev, Carsten Alsb and Søren Møller
• MicroRNAs (miRNAs) comprise a recently identified class of small, non-protein-coding regulatory molecules that play important roles in many physiologic and pathologic processes, including differentiation, viral infection, and oncogenesis.
• In cancer, abnormal miRNA expression suggests that these molecules may serve as valuable diagnostic and prognostic molecular signatures.
• To study the global miRNA expression profiles in breast cancer, we use the miRCURY™ LNA Array platform, which is based on locked nucleic acid (LNA)-modified capture probes that have uniquely high affinities for miRNA.
• For quantitative validation of new miRNA biomarkers, we apply a novel miRCURY™ LNA qPCR assay.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE