Microsatellites distinguish sexual vs. apomictic reproduction in spontaneous Rubus hybrids
Poster Jan 20, 2011
Lindsay V. Clark and Marie Jasieniuk
In previous work we identified spontaneous hybrids between the native, sexual R. ursinus and the non-native, apomictic R. armeniacus and R. pensilvanicus. Using microsatellite genotypes of seeds and seedlings, we determine that the hybrids produce a mix of sexual and clonal offspring, and that sexual seeds have higher viability. This has implications for the ability of hybrid Rubus to evolve into new invasive forms.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE