Mimetix® Electrospun Scaffolds for 3D Culture of Upcyte® and Primary Hepatocytes
Poster Sep 10, 2014
E. Heister, R. McKean (1) S. D. Ramachandran, B. Munst (2) B. Burkhardt, V. Haussling, A. Niissler (3)
This study investigates the Mimetix® electrospun scaffold, which consists of highly uniform electrospun PLLA microfibres supporting the three-dimensional growth of cells, as a cell-based assay system for upcyte® and primary hepatocytes. Migration into the scaffold, cell viability, as well as urea production and basal and induced CYP3A4 enzyme activity as typical hepatocyte functions were analysed. Both cell types were able to grow in the scaffolds for longer periods than in 2D. Primary hepatocytes also showed increased urea production in long-term cultures (2 weeks), while upcyte® Hepatocytes were responsive to Rifampicin, a proto-typical inducer of CYP 3A4 and showed higher basal, as well as induced levels of CYP activity than when grown in a 2D monolayer.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE