Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing
Poster Aug 30, 2005
Joe Graham, Sheila Fisher, Tracey Honan, Susan Faro and Aoife Gallagher
Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA (formerly the Whitehead Center for Genome Research). The Genome Sequencing and Analysis program at the Broad Institute places emphasis on the development of scalable methods employing automated laboratory procedures and informatics systems. The current rate of sequencing is over 40 million lanes per year, sequencing human, mouse, and other genomes.
This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as the world’s leading genome research institute.
The novel ‘K’ DNA extraction protocol offers a reliable alternative for preparing fecal specimens for 16S rRNA gene amplicon sequencing that maintains representation of microbial populations in a sample, with the added benefits that the K method reduces hands-on time by up to 20 x for 96 sample preparations.READ MORE