Mobility of Aeroallergens in Home: Effect of Location of Air Sampling and Implication for Evaluation of Patient Exposure
Poster Mar 05, 2016
Julian Gordon1, Paul Detjen, Andrea Wachter & Prasanthi Gandhi1
RATIONALE: We developed a user-friendly airborne allergen sampling device for patients with allergic asthma or rhinitis. This device was previously evaluated for its use in the bedroom. Here we run multiple devices at multiple locations within a house to determine whether bedroom aeroallergen profile is representative of the whole house.
METHODS:The devices were run at the same time at 12 locations in a home occupied by a family and a dog. The dog had access to all rooms except the basement. Twelve common household allergens were tested with MARIA™ multiplex immunoassays kits from Indoor Biotechnologies. Biomass measurements on the same samples were performed by spectrophotometry. Allergen and biomass capture increased linearly for 5 days and plateaued between 5 and 7 days. Five days was therefore selected as the standard sampling time.
RESULTS: Allergen profiles showed similar levels of Can f 1 (dog) at all testing locations except for a trace found in the basement from which the dog was restricted. Mold Asp f 1 was found in the basement but in no other location. Four locations showed timothy grass at borderline detectable levels. No other allergens were detected. Biomass was within a cv of +14%, showing relative consistency of air quality throughout the house.
CONCLUSIONS: Outdoor allergens may appear sporadically. Results are consistent with a dynamic equilibrium throughout the house depending on air flow and pet movements. Home aeroallergen sampling from any of several locations may accurately reflect a person’s allergen exposure.
High Speed Analysis: Combining Fast GC with Time-of-Flight Mass Spectrometry for Complex Sample Analysis in Under One MinutePoster
The demand for more efficiency and productivity in the laboratory has led to applications using Fast GC-MS methods for routine analysis in a wide range of fields.READ MORE
Idiopathic Pulmonary Fibrosis: The Importance of Multiple-site SamplingPoster
This research project attempts to highlight the importance of sampling from multiple sites of the lungs of patients with Idiopathic Pulmonary Fibrosis. Examining the histopathology of the human lung samples with IPF shows varying levels of fibrosis in different sites of the lung. To ensure an accurate representation of a patient's lungs, it is important to obtain samples from multiple sites on the lobes of the lungs.READ MORE
Hepatocellular Carcinoma with Small Cell Histology: A Rare Mimic of Metastatic Tumors Involving the LiverPoster
Two cases of hepatocellular carcinoma (HCC) with small cell histology, a rare morphologic variant of HCC, are presented.READ MORE
Comments | 0 ADD COMMENT
16th Annual Conference on Laboratory Medicine and Pathology
Sep 17 - Sep 18, 2018