Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLL
Poster Apr 21, 2015
Elisabeth Walsbyl, Paul Brennan', Guy Pratte, Andrea Buggins3, Tanja N Hartmann'', Chris Fegan1 and Chris Pepper'
We have recently developed a novel circulating model of chronic lymphocytic leukaemia (CLL) that mimics the transient interactions that take place between circulating lymphocytes and vascular endothelium. Here we show that both normal and malignant lymphocytes actively underwent transendothelial migration. Furthermore, seeding of CXCL12-secreting MRC5 cells into the extravascular space (EVS) resulting in significantly enhanced CLL cell migration (P = 0.024) but no increase in T-cell migration. Both CLL cells and T-cells recovered from the EVS showed evidence of activation markers and entry into cell cycle as demonstrated by increased Ki-67 expression (P<0.0001). We subsequently established that CLL cell Ki-67 expression was dependent on the presence of T-cells, as depletion of the T-cells from the circulating compartment significantly inhibited Ki-67 induction (P = 0.003). It is worthy of note that as a proportion of all circulating peripheral blood mononuclear cells, CD3+ T-cells migrated significantly more than CD19+ CLL cells (P = 0.02). This seems likely to be promoted by CLL cell secretion of T-cell attracting chemokines CCL3 (298pg/m1±197.5) and CCL4 (56pg/m1±115.6) in our model. Interestingly, CD38+ CLL samples showed elevated levels of CCL3 and CCL4 suggesting that increased T-cell recruitment may contribute to the inferior clinical outcomes seen in these patients. Taken together the data presented here highlight the requirement of T-cells in the pathology of CLL.
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
The Utility of Pediatric Asthma Severity Scores in Disposition Decisions for Status AsthmaticusPoster
We aimed to determine if the Pediatric Asthma Severity Score (PASS) predicts a patient's appropriate level of care in the inpatient setting, with special attention to patients who required rapid transfer to another level of care after initial disposition and/or hospital readmission.READ MORE
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Epigenetics in the nervous system: development and disease
Oct 01 - Oct 03, 2018