Modular Bench Top Automation
Poster Feb 15, 2012
Paul Held, Dean Mulyk, Lenore Buehrer and Grace Mangialardi
Using the modular ELISA workstation we have automated the assay process steps of an HIV ELISA assay kit. Plates with samples and controls can be loaded on to the system and the user simply presses a button and walks away. This poster will detail specifics of such a system, with automation workflows and details regarding throughput and performance provided.
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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer cluster
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