Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs
Poster Jan 14, 2013
Zeev Smilansky, Sima Barhoom, Ian Farrel, Dvir Dahary, Andrew Leask, Peter Vanderklish, Marcelo Ehrlich, Barry S. Cooperman and Orna Elroy-Stein
CHO cells were co-transfected with bulk Cy3- and Rho110- labeled yeast tRNAs. At 7h post transfection, cells were treated or not with puromycin or cycloheximide prior to fixation and imaging. CHO cells, infected with EHDV2-IBAV were co-transfected with Cy3 and Rho110 fl-tRNA. Results show typical triple co-localizations, consistent with the formation of ‘viral factories’.
We show that network analysis of co-localized ions from mass spectrometry imaging data provides a detailed chemo-spatial insight into the metabolic heterogeneity of tumors. Furthermore, module preservation analysis between colorectal cancer patients with and without metastatic recurrence suggests hypotheses on the nature of the different local metabolic pathways.READ MORE