Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs
Poster Jan 14, 2013
Zeev Smilansky, Sima Barhoom, Ian Farrel, Dvir Dahary, Andrew Leask, Peter Vanderklish, Marcelo Ehrlich, Barry S. Cooperman and Orna Elroy-Stein
CHO cells were co-transfected with bulk Cy3- and Rho110- labeled yeast tRNAs. At 7h post transfection, cells were treated or not with puromycin or cycloheximide prior to fixation and imaging. CHO cells, infected with EHDV2-IBAV were co-transfected with Cy3 and Rho110 fl-tRNA. Results show typical triple co-localizations, consistent with the formation of ‘viral factories’.
We show that network analysis of co-localized ions from mass spectrometry imaging data provides a detailed chemo-spatial insight into the metabolic heterogeneity of tumors. Furthermore, module preservation analysis between colorectal cancer patients with and without metastatic recurrence suggests hypotheses on the nature of the different local metabolic pathways.READ MORE
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE