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Multicolor Detection of CD45 on Human Monocytes with Quantum Dots in a High-Throughput Format

Poster   Feb 20, 2006

 
Multicolor Detection of CD45 on Human Monocytes with Quantum Dots in a High-Throughput Format
 
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Paul Wylie, John Budd and Marcel Bruchez

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.

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