Nanoliter Volume Pin Tool Transfers as Measured by a Dual-Dye Absorbance Method
Poster Feb 07, 2012
Duong T. Chau; Patrick H. Cleveland, Ph.D. (V & P Scientific); John Thomas Bradshaw, Ph.D. (ARTEL)
The V&P Pin Tool array was used to deliver aliquots of both aqueous-based and DMSO-based FITC sample solution into test plates under controlled experimental conditions. The same testing was conducted using the dual-dye absorbance approach implemented by the ARTEL Multichannel Verification System (MVS). Each measurement method was tested side-by-side on a series of pins capable of transferring from 20 to 315 nl. The effects of solvent composition, dye concentration, and source plate volume on actual delivered volume were compared for each system. The MVS based assays and fluorescence-based assays resulted in good general agreement. Areas of divergent results appear to be due to dye concentrations effects on the liquid surface tension or solvent/dye composition. Theories explaining these results are discussed.
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE