NanoQuot™, A Cost-Effective, Flexible Nanoliter Dispenser for High Throughput Screening
Poster Feb 01, 2007
Paul Held, Wendy Goodrich and Jason Greene
BioTek has developed the NanoQuot™, a dispenser capable of running stand-alone or computer controlled as part of a robotics system. Positive gas pressure system, in conjunction with eight precision valves, provides accurate and precise dispensing of volumes ranging from 100 nanoliters to 40 microliters in 96-, 384-, and 1536-well plates. The dispense head is adjustable for different plate heights, and removable, allowing the NanoQuot to be shared by multiple groups without the worry of contamination.
The NanoQuot’s low input gas pressure requirement allows house air, purified pressured gas, or a dedicated small compressor to be used. The instrument comes pre-calibrated with three liquid types and the end user can create new profiles with their liquids. Besides aqueous solutions, the NanoQuot can also handle viscous solutions and organic solvents. The user interface provides complete programming capabilities from the keypad. Information regarding design and performance, including accuracy and precision, will be provided.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE