Neuronal Mislocalization of Mutant DHHC9 in X-Linked Intellectual DIsability
Poster Feb 15, 2017
Lisa Kirouac, Krishna Reddy, Monic Amin and Robert Deschenes
DHHC9 belongs to a family of protein acyl transferase (PAT) enzymes. PATs enzymatically add fatty acid palmitate to cysteine residues on specific protein substrates resulting in increased hydrophobicity and membrane association. This modification is reversible by the action of depalmitoylating thioesterases. There are over 300 candidate palmitoylated proteins identified in rat cortical neurons, suggesting that this dynamic process plays a key role in the spatiotemporal distribution of proteins within the neuron. Neurons are polarized cells with discrete protein domains and DHHC9 and other PATs facilitate proper trafficking of proteins to these domains. Recently, mutations in the zDHHC9 gene have been identified in individuals with X-Linked Intellectual Disability (XLID). Here, we characterize a specific XLID nonsense mutation (*R298) in the zDHHC9 gene that results in the expression of a C-terminal truncated protein, in the context of the mature hippocampal neuron.
In order to generate a robust protocol for MEA recording on hiPSC- derived neurons, we evaluated several conditions, which could affect culture performance (1.neuron seeding density; 2.seeding medium; 3.astrocyt eco-culture). These conditions were evaluated with BrainXell’s hiPSC-derived spinal motor neurons, cortical glutamatergic neurons and mixed cortical neurons.READ MORE