Neuroprotection by T-Lymphocytes and Stem Cells After Ischemic Stroke
Poster Feb 13, 2017
Elliot Neal, MS; Sandra Acosta, MS PhD; Yuji Kaneko, PhD; Cesario Borlongan, MA PhD
Stroke is the second leading cause of death worldwide and the third leading cause of adult disability in adults. Ischemic stroke triggers an inflammatory response in the brain that is cytotoxic. In response to ischemic stroke, T-cells from mobilize to the brain and modulate both cytotoxic and protective inflammation. Regulatory T (Treg)-cells exert a neuroprotective effect after ischemic stroke by inhibiting both inflammation and cytotoxic T-cell activation. Transplantation of bone marrow-derived stem cells (BMSCs) after ischemic stroke has a neuroprotective effect. One way that BMSCs protect neurons from apoptosis is by attenuating innate inflammation, but response of the adaptive immune system has not been well-studied. Our lab has found that implanted stem cells accumulate in locations with known importance to the adaptive immune system like the spleen. In this study, regulatory T-cells and BMSCs were shown to be neuroprotective following ischemic treatment of primary rat neurons.
In order to generate a robust protocol for MEA recording on hiPSC- derived neurons, we evaluated several conditions, which could affect culture performance (1.neuron seeding density; 2.seeding medium; 3.astrocyt eco-culture). These conditions were evaluated with BrainXell’s hiPSC-derived spinal motor neurons, cortical glutamatergic neurons and mixed cortical neurons.READ MORE