Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Poster Oct 05, 2011
Oksana Sirenko, Susan DeLaura, Lucas Chase, Jayne Hesley and Evan F. Cromwell
Human neurons derived from induced pluripotent stem cells (iPSC), such as iCell® Neurons are very attractive for such studies because they exhibit functionality and behavior of mature neurons and are available in large quantities. Live cell assays can be automated with the ImageXpress® Micro system and MetaXpress® software to allow characterization of neurons and neuronal networks for health and extent of the network.
This poster presents examples of the use of these systems to study inhibition of neuronal development and neurotoxicity in assays that are well suited for screening of lead compounds and potentially important for reducing animal experimentation and cost of pre-clinical development.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Microfluidic Extrusion of Cell-laden Hydrogel Fibers for 3D-BioprintingPoster
An overview on how the incorporation of microfluidic platforms in 3D-bioprinters can open new routes in the creation of heterogeneous, tissue-specific bio-constructs and in the high-throughput screening of bioinks compositions for regenerative medicine and in vitro tissue modeling.READ MORE
Use of Non-modified RNAs for the Derivation of Clinically-relevant iPS Cell Lines from Human Blood, Urine and Skin Cells using GMP-compliant ReagentsPoster
We have developed protocols using our StemRNA-NM Reprogramming Kit for generating iPS cells from skin-derived fibroblasts, blood-derived EPCs, and urine-derived UDCs using GMP-compatible reagents (NutriStem medium, iMatrix-511 recombinant laminin). These protocols generate GMP-compatible stem cells without the use of feeders, conditioned medium, additional small molecules or proteins, or passaging during reprogramming.READ MORE