Neutrophil Adhesion: A HCS Compatible Assay Using the Acumen eX3
Poster Jul 05, 2011
Diana Caracino and Paul Wylie
The Acumen eX3 can be used to study the process of cellular adhesion, whereby adherent cells types specifically, endothelial cells can be grown to confluence in microtitre plate wells and other cells types e.g. neutrophils added. The neutrophils can be differentially stained with calcein AM and the adhesion profile monitored and quantified. Cell adhesion can be determined simply by correlating retained fluorescence with cell number.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE