New 3D Black Substrate for Protein Microarrays with Improved Dynamic Range
Poster Jan 03, 2006
Markus Hollas, Eric Jallerat, Karl Pflanz, Inka Praulich, Johanna Walter
IntroductionSurge in proteomics research has accelerated the development of new protein arrays as an indispensable tool to study high density protein interaction. The most important factor that determines the performance of protein microarrays is the substrate on which the proteins are immobilised. The microarray substrate, used either in an interaction or capture mode has to provide several key functions. High binding capacity for any proteins without changing their biologically active conformation as well as a low auto fluorescence in order to generate a high signal to noise ratio are essential. This enables the detection of the interaction or capture event within a widedynamic range.
A Microarray slide combining the benefits of increased surface area of 3D cellulose nitrate with the excellent low auto fluorescence of a black substrate has been developed and evaluated. The 3D structure was designed to offer a high binding affinity for proteins and to better stabilize their active conformation even in presence of denaturing temperature conditions. A competitive study analysing the background fluorescence and the detectable concentration range of a chosen antigen is presented. An Affymetrix Spotting roboter 417 arrayer was used for protein dotting. The analysis is based on fluorescence scanning experiments with an Affymetrix 428 array scanner at a gain of 60. Data analysis was made with ImaGene Premium 5.5.3 by Biodiscovery Inc.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Functional Analysis of RAD51B and RAD23B SNPs using Circular Chromosome Conformation Capture (4C) in Human Prostate Cell LinesPoster
The aim of this study is to understand the mechanism behind the association between single nucleotide polymorphisms (SNPs), lying in non-coding regions near or within RAD51B and RAD23B, and prostate cancer risk by analysing their interactions with the whole genome using Circular Chromosome Conformation Capture (4C).READ MORE
Preparation of Photonic Biosensors by Inkjet Printing TechnologyPoster
In order to enable local functionalization of label-free optical waveguide biosensors in a cost effective mass-fabrication compatible manner, we investigate surface modification employing inkjet printing of functional polymers and UV-curable benzophenone dextran.READ MORE