New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples
Poster May 12, 2014
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji
T helper cells (Th cells) play important roles in regulating the responses of the immune system. They secrete cytokines to stimulate various effector cells, such as cytotoxic T cells, B cells and macrophages. Accurate measurement of Th cytokine expression is critical to identify the corresponding Th cell responses and to develop in depth understanding of the immune responses. We have developed two multiplexed assay panels, i.e., the human and mouse Th panels, using fluorescence–encoded beads that are suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-γ and TNF-α, which are collectively secreted by Th1, Th2, Th9, Th17, Th22 or T follicular cells. Each antibody pair was carefully selected for assay specificity, sensitivity, accuracy and reproducibility. The assays provide higher detection sensitivity and broader dynamic ranges than traditional ELISA methods. The panels have been validated by detecting expected changes in biological samples. Further advantages include small sample volume, flexible assay configurations, and time- and cost-effectiveness. The Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.
Quantitative Cell-Based Bioassays for Individual and Combination Immune Checkpoint Immunotherapy TargetsPoster
The human immune system is comprised of a complex network of immune checkpoint receptors that are promising new immunotherapy targets for the treatment of a variety of diseases including cancer and autoimmune-mediated disorders.READ MORE
Reporter Bioassays to Assess Therapeutic Antibodies for Immunotherapy ProgramsPoster
Immunotherapy, also called biologic therapy or biotherapy, stimulates certain parts of the immune system to fight diseases such as cancer. Important drug targets in immunotherapy include: Co-inhibitory receptors, such as PD-1/PD-L1, CTLA-4, LAG3, Tim3; and co-stimulatory receptors, such as GITR, CD40, OX40, 4-1BB.
Current approaches to assaying these targets are cumbersome and variable. Here we offer an improved in vitro bioassay approach.
Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLLPoster
We have recently developed a novel circulating model of chronic lymphocytic leukaemia (CLL) that mimics the transient interactions that take place between circulating lymphocytes and vascular endothelium. Here we show that both normal and malignant lymphocytes actively underwent transendothelial migration.READ MORE