New Platforms and Systems for DNA Microarrays
Poster Feb 01, 2007
B. Henze, B. Saal, D. Drutschmann, K. Wellesen and P. Schüßler
DNA microarrays are commonly used in expression profiling and genetic analysis experiments. Today still many DNA arrays are based on short oligonucleotides or cDNAs. These can provide either high specificity or high sensitivity respectively, but cannot combine these qualities. Operon has designed probes of different lengths to various positions in the Open Reading Frames (ORFs) and the results clearly show that 70mers offer the optimal combination of specificity and sensitivity.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE