New Platforms and Systems for DNA Microarrays
Poster Feb 01, 2007
B. Henze, B. Saal, D. Drutschmann, K. Wellesen and P. Schüßler
DNA microarrays are commonly used in expression profiling and genetic analysis experiments. Today still many DNA arrays are based on short oligonucleotides or cDNAs. These can provide either high specificity or high sensitivity respectively, but cannot combine these qualities. Operon has designed probes of different lengths to various positions in the Open Reading Frames (ORFs) and the results clearly show that 70mers offer the optimal combination of specificity and sensitivity.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE