Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
Poster Dec 04, 2014
SC Cribbes, S Brightwell, K Kaji
Current methodologies for the detection of inducible Pluripotent Stem (iPS) reprogramming are either disruptive e.g. flow cytometry (FC) or low in throughput e.g. fluorescent microscopy (FM). Using the Celigo S Imaging Cytometer and secondary iPS reprogramming we have developed a methodology that combines the advantages of both flow cytometry and fluorescent microscopy. This approach is based on the fluorescent identification of iPS colonies that express the four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc, by expression of mOrange placed after the four factors following ires and the progress of reprogramming using fluorescent detection of the pluripotency reporter Nanog-GFP+ cells within these colonies. This method can be used to not only follow the reprogramming kinetics but could also be used to examine the effect of extrinsic factors, thus, providing a strong tool to investigate molecular mechanisms of reprogramming.
Nexcelom’s Celigo S imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming . Using f-theta optics, Celigo provides high quality, whole well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provides quantitative output of iPS reprogramming based on mOrange and GFP fluorescent colony detection.
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
The Utility of Pediatric Asthma Severity Scores in Disposition Decisions for Status AsthmaticusPoster
We aimed to determine if the Pediatric Asthma Severity Score (PASS) predicts a patient's appropriate level of care in the inpatient setting, with special attention to patients who required rapid transfer to another level of care after initial disposition and/or hospital readmission.READ MORE
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2nd Annual Artificial Intelligence in Drug Development Congress
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