Non-invasive Liquid Biopsy
We have developed Dynabeads® magnetic beads for enrichment of circulating tumor cells (CTCs), both for positive and negative selection. For a positive selection Dynabeads® magnetic beads pre-coupled with EpCAM (epithelial cell adhesion molecule) were used to capture circulating epithelial cells. For negative selection, a combination of Dynabeads® CD45 and Dynabeads® CD15 gave a 95 - 99 % depletion of mononuclear cells.
In addition, we have developed Dynabeads® magnetic beads for capture of exosome sub-populations from pre-enriched cell culture supernatant, by coupling Dynabeads® with antibodies targeting CD81, as well as CD9 and CD63 (data not shown).
Finally, we aim to provide a universal solution for high-throughput automation for efficient isolation of circulating cell-free DNA (cfDNA) isolation from large volumes of serum or plasma by using Dynabeads® MyOneTM SILANE. The bead surface chemistry in combination with the Proteinase K-free workflow enables highly efficiency capture of DNA in the <220 base pair range compared to other methods, providing the added advantage of a quick, reproducible, and cheap enrichment of circulating cfDNA.