Non-invasive Liquid Biopsy
Poster Mar 20, 2015
Frauke Henjes1, Lise Aagaard1, Darren Ellis1, Hannah Lindstroem1, Morten Oksvold2,3, Axl Neurauter1, Ketil W. Pedersen1.
A non-invasive liquid biopsy permits the analysis of multiple circulating biomarkers in one sample and facilitates the discovery of disease as well as treatment response monitoring in applications such as oncology and prenatal analysis.
We have developed Dynabeads® magnetic beads for enrichment of circulating tumor cells (CTCs), both for positive and negative selection. For a positive selection Dynabeads® magnetic beads pre-coupled with EpCAM (epithelial cell adhesion molecule) were used to capture circulating epithelial cells. For negative selection, a combination of Dynabeads® CD45 and Dynabeads® CD15 gave a 95 - 99 % depletion of mononuclear cells.
In addition, we have developed Dynabeads® magnetic beads for capture of exosome sub-populations from pre-enriched cell culture supernatant, by coupling Dynabeads® with antibodies targeting CD81, as well as CD9 and CD63 (data not shown).
Finally, we aim to provide a universal solution for high-throughput automation for efficient isolation of circulating cell-free DNA (cfDNA) isolation from large volumes of serum or plasma by using Dynabeads® MyOneTM SILANE. The bead surface chemistry in combination with the Proteinase K-free workflow enables highly efficiency capture of DNA in the <220 base pair range compared to other methods, providing the added advantage of a quick, reproducible, and cheap enrichment of circulating cfDNA.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE