Novel, Fully Automated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq
Poster Apr 30, 2012
Collaborative research by Pioneer Hi-Bred (a DuPont company) and Azure PCR Limited
qPCR is extensively applied to determine the identity of genetically modified (GM) seeds and plants. Analysis and interpretation of qPCR data for any application is limited by sample variability1, poor assay performance1 and arbitrarily set thresholds2 which can all lead to ambiguous and subjective result calling that is reliant upon the expertise and experience of the scientist interpreting the assay. Processing of raw data output from thermal cyclers using analysis software, to provide data more easily interpretable by the human eye, can lead to loss of data3 and error prone sample calling4,5. In this study we retrospectively examined data produced in qPCR-based plant genotyping tests at Pioneer Hi-Bred to assess if patent-pending AzurePCR automated analysis produced similar results without the need for manual input of analysis parameters or manipulation of raw data.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s DiseasePoster
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.READ MORE
Characterizing GPCR Activation Using Automated Live Cell ImagingPoster
Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software.READ MORE