Novel, Fully Automated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq
Poster Apr 30, 2012
Collaborative research by Pioneer Hi-Bred (a DuPont company) and Azure PCR Limited
qPCR is extensively applied to determine the identity of genetically modified (GM) seeds and plants. Analysis and interpretation of qPCR data for any application is limited by sample variability1, poor assay performance1 and arbitrarily set thresholds2 which can all lead to ambiguous and subjective result calling that is reliant upon the expertise and experience of the scientist interpreting the assay. Processing of raw data output from thermal cyclers using analysis software, to provide data more easily interpretable by the human eye, can lead to loss of data3 and error prone sample calling4,5. In this study we retrospectively examined data produced in qPCR-based plant genotyping tests at Pioneer Hi-Bred to assess if patent-pending AzurePCR automated analysis produced similar results without the need for manual input of analysis parameters or manipulation of raw data.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.