Oligonucleotide Purification Strategies using a New High-Capacity Anion Exchange Resin
Poster Jan 05, 2007
M. Deetz, J. Fisher, A. Gehris, J. Maikner and M. Kinzey
With the advent of nucleic acid silencing technologies and the need for high purity diagnostic and therapeutic oligonucleotides, there is a need for high-capacity chromatographic supports that can deliver economic purification processes.
A new, 30 micron, mono-sized, polymeric resin has been recently developed that provides high resolution and high capacity for synthetic oligonucleotides. Physical properties of this new resin will be described, including particle size uniformity, ion exchange capacity, pressure stability, and chemical stability.
The purification of two synthetic 12mer DNA oligonucleotides (AAA CCT GAT GTG and GGT GGT GGT GGT) will be demonstrated, and will compare yields and purities to those from commercially available anion exchange resins.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.