Optical Microchip Sensors for Multiplexed Detection of Biological Pathogens
Poster Jul 06, 2011
D. Bhatta, A. Michel, M. Marti Villalba, G. D. Emmerson, I. J. G Sparrow, M. B. McDonnell, E. A. Perkins , R. W. Ely and G. A. Cartwright
Sensitivity to biological agents is conferred by functionalising individual sensing regions with different antibodies selected against various targets of interest. Antigen binding to the surface-immobilised antibodies results in localised changes in refractive index, which manifest as increases in wavelength of light reflected from the sensor chip. Real-time detection of multiple bio-hazardous agents including bacterial cells/spores, viruses and toxins has been demonstrated. This multi-analyte capability, coupled with its inherent robustness, portability and ease of use highlights the potential use of SpectroSens technology in applications ranging from bio-hazard detection for security and defence purposes to point-of-care medical diagnostics.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE