Optimisation Workflow for the Production and Processing of High Quality Microarrays Applications in DNA, Peptide, antibody, and Carbohydrate Microarraying

Introduction

Task
Preparation and processing of microarrays for DNA, peptide, antibody, and carbohydrate applications

Problem
To obtain data of highest quality and meaningfulness a careful optimisation of protocols is necessary. Experimental errors need
to be reduced as much as possible.

Solution
We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.