Optimisation Workflow for the Production and Processing of High Quality Microarrays Applications in DNA, Peptide, antibody, and Carbohydrate Microarraying
Poster Nov 10, 2005
Jens Sobek, Catharine Aquino and Ralph Schlapbach
Preparation and processing of microarrays for DNA, peptide, antibody, and carbohydrate applications
To obtain data of highest quality and meaningfulness a careful optimisation of protocols is necessary. Experimental errors need
to be reduced as much as possible.
We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.
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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE