Optimisation Workflow for the Production and Processing of High Quality Microarrays Applications in DNA, Peptide, antibody, and Carbohydrate Microarraying
Poster Nov 10, 2005
Jens Sobek, Catharine Aquino and Ralph Schlapbach
Preparation and processing of microarrays for DNA, peptide, antibody, and carbohydrate applications
To obtain data of highest quality and meaningfulness a careful optimisation of protocols is necessary. Experimental errors need
to be reduced as much as possible.
We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE