Optimized Workflow for Single Cell Copy Number Profiling Using High Resolution Oligo CGH Arrays
Poster Nov 10, 2016
Scott Basehore, Paula Costa, Natalia Novoradovskaya, Anniek De Witte, Stephanie Fulmer-Smentek
The ability to characterize individual genomes in single cells is very important in pre-implantation genetics research. Traditional FISH and PCR based techniques, and more recently BAC arrays, have been used to provide insights into a single cell’s genome, with low resolution due to the limited number of loci that can be analyzed simultaneously. Here we describe GenetiSure Pre-Screen, a same day, cost-effective, analysis workflow (Figure 1) that combines whole genome amplification (WGA) with copy number (CN) profiling using high-resolution oligo CGH microarrays.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE