Optimized Workflow for Single Cell Copy Number Profiling Using High Resolution Oligo CGH Arrays
Poster Nov 10, 2016
Scott Basehore, Paula Costa, Natalia Novoradovskaya, Anniek De Witte, Stephanie Fulmer-Smentek
The ability to characterize individual genomes in single cells is very important in pre-implantation genetics research. Traditional FISH and PCR based techniques, and more recently BAC arrays, have been used to provide insights into a single cell’s genome, with low resolution due to the limited number of loci that can be analyzed simultaneously. Here we describe GenetiSure Pre-Screen, a same day, cost-effective, analysis workflow (Figure 1) that combines whole genome amplification (WGA) with copy number (CN) profiling using high-resolution oligo CGH microarrays.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE