Optimized Workflow for Single Cell Copy Number Profiling Using High Resolution Oligo CGH Arrays
Poster Nov 10, 2016
Scott Basehore, Paula Costa, Natalia Novoradovskaya, Anniek De Witte, Stephanie Fulmer-Smentek
The ability to characterize individual genomes in single cells is very important in pre-implantation genetics research. Traditional FISH and PCR based techniques, and more recently BAC arrays, have been used to provide insights into a single cell’s genome, with low resolution due to the limited number of loci that can be analyzed simultaneously. Here we describe GenetiSure Pre-Screen, a same day, cost-effective, analysis workflow (Figure 1) that combines whole genome amplification (WGA) with copy number (CN) profiling using high-resolution oligo CGH microarrays.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Young Adult Women’s Relationship Status and HPV Risk Perceptions: A Barrier to HPV Vaccination?Poster
Human papillomavirus (HPV) vaccine rates continue to be low in the United States. Young women ages 18-26 years are eligible for catch-up vaccination but previous research shows that relationships status and percieved risk may be barriers to HPV vaccination. The purpose of this quantitative study was to assess the association between relationship status and perceived risk for HPV among young adult women.READ MORE
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE