Overcoming the Challenges of Analysing Ionic Polar Pesticides in Food
Poster Nov 03, 2017
Benjamin Wuyts, Euan Ross, Dimple Shah, Simon Hird, Gareth Cleland.
Glyphosate, a non-selective broad spectrum herbicide, accounts for more than half of global herbicide sales. While discussions on the toxicological concerns of glyphosate and associated compounds continue, maximum residue limits (MRLs) are enforced globally, requiring continued analytical testing to ensure consumer safety. In previous work, various methodologies have been presented, looking at underivatised options for the direct analysis of polar pesticides in food. [Scan the QR code below to discover more] Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised analysis of glyphosate and a selection of other anionic pesticides. Separation is achieved under Hydrophilic Interaction Chromatography (HILIC), after a simple column conditioning to activate the mechanism. Preliminary method performance, in the absence of isotopically labelled internal standard is summarised, in accordance with relevant SANTE 11945/2015 guidelines.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological SamplesPoster
Automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites.