Pathways for Biological Reagent Quality and Workflow Tracking (CIMS)
Poster Feb 01, 2007
Biological reagents are the single most important factor in the success of assays. Use of poor or unsuitable biological materials can lead to huge wastage of time and resources. CIMS is a novel system that provides an unique combination of active tracking in the laboratory and desktop analysis software. It provides laboratories with easy-to-use data portals for gathering cell quality data such as cell viability, cell density and passage number. It associates these reagent quality data with plates and tracks their progress around the lab. Once assay screening has been performed, the screening results for plates are merged with the reagent quality data. The CIMS viewer application can then be used to quickly and easily identify whether poor or unexpected screening results were caused by biological factors.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE