Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation System
Poster Apr 07, 2017
Lidia Prieto-Lafuente1, Claire MacDonald1, Emma Brown1, Rainer Nitsche2 and Alex Siebold3
1)Agilent Technologies UK Ltd., Edinburgh, UK. 2) Agilent Technologies, Waldbronn, Germany 3) Agilent Technologies, Inc., La Jolla, CA, USA
The Agilent 4200 TapeStation system provides automated, fast, and reliable DNA and RNA electrophoresis for up to 96 samples using prepackaged reagents and minimal manual handling. The Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape assays have been developed for the analysis of DNA fragments (100 bp to 5,000 bp) within the Next Generation Sequencing (NGS) workflow. Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays. In addition, the performance was compared to the Agilent 2100 Bioanalyzer and the Agilent 2200 TapeStation system.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE