Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation System
Poster Apr 07, 2017
Lidia Prieto-Lafuente1, Claire MacDonald1, Emma Brown1, Rainer Nitsche2 and Alex Siebold3
1)Agilent Technologies UK Ltd., Edinburgh, UK. 2) Agilent Technologies, Waldbronn, Germany 3) Agilent Technologies, Inc., La Jolla, CA, USA
The Agilent 4200 TapeStation system provides automated, fast, and reliable DNA and RNA electrophoresis for up to 96 samples using prepackaged reagents and minimal manual handling. The Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape assays have been developed for the analysis of DNA fragments (100 bp to 5,000 bp) within the Next Generation Sequencing (NGS) workflow. Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays. In addition, the performance was compared to the Agilent 2100 Bioanalyzer and the Agilent 2200 TapeStation system.
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Early life stress (ELS) is highly associated with development of psychopathology
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Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
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Establishment and succession of the human microbiome begins at birth and microbial composition adapts alongside human development and growth. Development of a healthy signature microbial community has significant effects on health.This study followed 18 very low birth weight (VLBW) infants, measured initially in the from Neonatal Intensive Care Unit (NICU), at the age of two to three years old.READ MORE