The Agilent 4200 TapeStation system provides automated, fast, and reliable DNA and RNA electrophoresis for up to 96 samples using prepackaged reagents and minimal manual handling. The Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape assays have been developed for the analysis of DNA fragments (100 bp to 5,000 bp) within the Next Generation Sequencing (NGS) workflow. Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays. In addition, the performance was compared to the Agilent 2100 Bioanalyzer and the Agilent 2200 TapeStation system.