Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation System
Poster Apr 07, 2017
Lidia Prieto-Lafuente1, Claire MacDonald1, Emma Brown1, Rainer Nitsche2 and Alex Siebold3
1)Agilent Technologies UK Ltd., Edinburgh, UK. 2) Agilent Technologies, Waldbronn, Germany 3) Agilent Technologies, Inc., La Jolla, CA, USA
The Agilent 4200 TapeStation system provides automated, fast, and reliable DNA and RNA electrophoresis for up to 96 samples using prepackaged reagents and minimal manual handling. The Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape assays have been developed for the analysis of DNA fragments (100 bp to 5,000 bp) within the Next Generation Sequencing (NGS) workflow. Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays. In addition, the performance was compared to the Agilent 2100 Bioanalyzer and the Agilent 2200 TapeStation system.
The novel ‘K’ DNA extraction protocol offers a reliable alternative for preparing fecal specimens for 16S rRNA gene amplicon sequencing that maintains representation of microbial populations in a sample, with the added benefits that the K method reduces hands-on time by up to 20 x for 96 sample preparations.READ MORE