Phytochip: a New Tool to Study the Diversity of Toxic Phytoplankton in the Bay of Seine
Poster Mar 05, 2013
Noyer Charlotte, Anton Leberre Véroniqueand Dreanno Catherine
Detection of harmful algal blooms (HABs), also called red tides, has become a challenging concern due to the direct impacts on public health and economy. Current methods consist in microscopic identification and enumeration of the cells of interests. However it is time-consuming, tedious and requires expert taxonomists. Advances in molecular biology allow the development of new tools. In this context, we aimed at developing a new DNA microarray: the phytochip. It should be a rapid and accurate method dedicated to the identification of the main toxic phytoplankton species in French waters.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE