Picking the Best CRISPR-Cas9 Targets for Functional Gene Knockout: A Machine Learning Algorithm Based on Both Specificity and Functionality
Poster Sep 24, 2015
Shawn McClelland, Emily M. Anderson, Žaklina Strezoska, Elena Maksimova, Annaleen Vermeulen, Steve Lenger, Tyler Reed, and Anja van Brabant Smith Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
Functional gene knockout is an important tool for understanding a gene's role in a system or for specifically manipulating a known system to achieve a desired outcome. Not all gene cleavage events result in functional knockout of the target protein. Here we share important advancements that have helped to achieve the goal of picking the best crRNA targets for functional gene knockout, and not just formation of indels (insertions or the deletions of bases in the DNA).
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE