Polarized Cell Expansion and Heterochronic Shift in Angiosperm Spermatogenesis
Poster Apr 04, 2012
Said Hafidh, Katarina Breznenova, Jana Fecikova, Vera Capkova and David Honys
Single cell expansion of the growing pollen tube and the coordinated control of germ cell division and sperm cell fate specification, are landmark features of the developing male gametophyte. We applied the Agilent 44K tobacco gene chip to conduct the first transcriptomic analysis of the tobacco male gametophyte. We have performed a comparative study with the Arabidopsis root-hair trichoblast transcriptome to evaluate genetic factors and common pathways in polarized cell-tip expansion. Extension of our analysis beyond the second haploid mitosis allowed construction of a genetic model likely to have instigate the heterochronic shift in Angiosperm spermatogenesis.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.