Polymer Microarrays for Biomaterial Development
Poster Apr 01, 2015
Simmonte, M.J.1, Dhaliwal, K.2, Cuschieri, K.3, Graham, S.V.4, Bradley, M.1
Polymer biomaterials have truly become integrated into modern day healthcare and medical science. These range from the basic, such as culture equipment and blood bags, to the complex, stimuli-dependent hydrogels, and leukocyte filters. Polymer microarrays have greatly improved the discovery of biologically relevant interactions by interrogating a chosen biological material against a library of polymers, simultaneously. In this work, the polymer microarray approach is applied in two different biological contexts to identify promising candidates for biomaterial development, as well as expanding the repertoire of polymer microarray application. Specifically the identification of polymer substrates for: the scavenge of DNA-based DAMPs in a blood based application; and for the enrichment of cancerous and precancerous cervical cells for improved cytology of PreservCyt®-treated LBC samples, has been investigated.
Using this approach, we have identified a number of poly(acrylate), poly(acrylamide), and poly(urethane) candidates that, while resistant to blood cell attachment, were capable of binding to mtDNA and nDNA. A second investigation has also elucidated substrates with preferential binding for fixed cervical cell lines, including the precancerous W12 model. These findings demonstrate the versatility of this experimental approach by providing some of the first examples of molecule-polymer and preserved cell-polymer interactions, identified via microarray.
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
Liver Transplantation for Hepatocellular Carcinoma Outperform Other Populations in the New Hepatitis C EraPoster
Long term results of HCC liver transplants (LTx) in the recent era of direct-acting antivirals (DAA) against hepatitis C (HCV) are not available. We aimed to examine the access, treatments and cure rates with DAA’s utilized in HCC LTx with HCV. Secondarily, we aimed to determine the long term impact of the Liver Cancer Program and DAA therapies on survival of HCC LTx.READ MORE