Polymerized Poly(ethylene glycol) Diacrylate Microfluidic Membrane Valves
Poster Mar 06, 2014
Chad I. Rogers, Joseph B. Oxborrow, Long-Fang Tsai, Gregory P. Nordin, and Adam T. Woolley
Polymerized poly(ethylene glycol) diacrylate (poly-PEGDA) was fabricated into pneumatically controlled, non-elastomeric membrane valves as a nonspecific adsorption resistant alternative to polydimethylsiloxane valves. Temporal response, valve closure, and long-term durability of these poly-PEGDA valves were evaluated. Valve operation up to 8 Hz was achieved with a ~100 ms opening time (without applied vacuum) and a ~20 ms closure time. Valve closure demonstrates less leakage in geometries with a larger membrane contact area (>0.3 mm2) and pedestals wider than 15 μm. After ~1000 actuations to reconfigure polymer chains and increase membrane elasticity, the fluid pressure needed to open a closed valve becomes the same as the applied control pressure. After this initial conditioning, the poly-PEGDA valves maintain a linear relationship between the closure pressure and the opening pressure required to initiate flow even after 115,000 actuations. These robust valves have potential for application as peristaltic pumps and in small volume bioassays or impedance detection.
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE