Polyphasic Approach to the Taxonomy of Thirteen Clostridium Strains by Means of Multivariate Data Analysis: Strategy for Describing a new Species
Poster Dec 20, 2006
ZR. Suárez, B. Cháves, FA. Aristizábal and D. Montoya
Polyphasic taxonomy is aiming at the integration of different kinds of data (i.e. phenotypic, genotypic and phylogenetic) on microorganisms and essentially indicates a consensus type of taxonomy. In this work multivariate data analysis was used as a tool for determining the taxonomy of thirteen solventogenic strains of Clostridia isolated from Colombian soils. This strategy was selected due to its ability for establishing relationships between individuals (strains) based on its characteristics (variables).
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE