Population Characterization of Brazilian Isolates of Ceratocystis spp. Using Microsatellites
Poster Jan 12, 2015
Edson Luiz Furtado, Ana Carolina Firmino, Michael Mbenoun, Denise Nakada Nosaki, Ariska Van der Nest, Jolanda Roux, Irene Bernes, Mike Wingfield
Ten SSR markers developed previously were used and were polymorphic. These markers produced a total of 76 alleles for the 49 C. fimbriata isolates used in this study. Three distinct populations were identified using STRUCTURE and LNK analyses. Analysis of population differentiation show a Ө = 0.27 and p ≤ 0.04. High values of genotypic diversity were obtained for the combined (68.25%) and separate populations (77.77%, 52.08% and 100%). For the combined population, the Association Index (Ia) and baD values (Rd) were 1.91 and 0.22, respectively, indicating a high rate of recombination among the isolates.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE