Population Characterization of Brazilian Isolates of Ceratocystis spp. Using Microsatellites
Poster Jan 12, 2015
Edson Luiz Furtado, Ana Carolina Firmino, Michael Mbenoun, Denise Nakada Nosaki, Ariska Van der Nest, Jolanda Roux, Irene Bernes, Mike Wingfield
Ten SSR markers developed previously were used and were polymorphic. These markers produced a total of 76 alleles for the 49 C. fimbriata isolates used in this study. Three distinct populations were identified using STRUCTURE and LNK analyses. Analysis of population differentiation show a Ө = 0.27 and p ≤ 0.04. High values of genotypic diversity were obtained for the combined (68.25%) and separate populations (77.77%, 52.08% and 100%). For the combined population, the Association Index (Ia) and baD values (Rd) were 1.91 and 0.22, respectively, indicating a high rate of recombination among the isolates.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE