Protein Expression Profiling with High-Content Antibody Arrays using Universal Linkage System ULS™ Protein Labeling
Poster Oct 10, 2005
Frank Opdam, Saskia Schoenmakers, Marie Lacombe, Sandra van der Locht, Alain van Gool and Paul van der Hoeven
Introduction• The Universal Linkage System (ULS™) is a platinum-based technology that allows direct labeling of proteins and nucleic acids with a large variety of labels and haptens, including many fluorophores, biotin, and dinitrophenol.
• In proteins, ULS™ forms a stable coordinative bond with the sulfur atom in Methionine and free Cysteine, and with a nitrogen in Histidines (Fig. 1).
• Is an easy and robust labeling method
• Provides high coverage of proteome
• Is stable in aqueous solution
• Labeling is compatible with a wide range of buffers and detergents including Tris, Glycine, SDS, Triton X-100, NP-40 etc.
Bioluminescent Kinase Profiling Systems For Characterizing Small Molecule Kinase InhibitorsPoster
In order to profile compounds against a broad panel of kinases, in-house profiling requires rigorous kinase assay development.READ MORE
Immunotherapy on Chip: “Under the Microscope” Drug-induced Modulation of the Immune ResponsePoster
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE