Putative Genes Identified on Two Growth Conditions of G. boninense
Poster Jan 10, 2015
Jayanthi N, Abrizah O, Low ETL, O-Abdullah M, Hogan M, Cuomo CA, Desjardins C, Abdul Manaf MA, Rajinder S, Birren B and Ravigadevi S
Ganoderma boninense is an infamous facultative fungal pathogen that has been the causal agent of basal stem rot (BSR) disease in oil palm (Elaeis guineensis). The disease has caused the deaths of palms as young as 12-24 months and has been a serious threat to the oil palm industry in South East Asian countries, especially Malaysia and Indonesia. The acreage of plantation infected with BSR disease in Peninsular Malaysia was recorded to be about 400,000 hectares. Fundamental studies on G. boninense are limited, especially on the genes involved in pathogenicity. Therefore, to further understand the fundamental biology of the genes involved in degradation, the mycelial of G. boninense was cultivated on two different mediums, fresh potato dextrose agar (PDA) in petri plates as the standard growth media (PER71) and rubber wood blocks (PER71-47) as the comparative.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE