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Pyroptosis Detection via Multiplexing Cell Death and Bioluminescent Caspase-1 Activity Assessments

Poster   Jul 14, 2017

 
Pyroptosis Detection via Multiplexing Cell Death and Bioluminescent Caspase-1 Activity Assessments
 
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Martha O’Brien1, Mike Scurria2, Tim Ugo2, Laurent Bernad2, James Cali1, and Dan Lazar1

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Multiplexing Cell-Based Assays Using 3D Culture Models

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Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers

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Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.

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Syringe Filters – How To Choose the Optimal Housing and Membrane Material for Your Application

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