Quality Monitoring on Human 14K Oligo Chips at the University of Calgary
Poster Feb 01, 2007
Xiuling Wang, Stephen M. Robbins and Mayi Arcellana-Panlilio,
The production of microarrays needs to be carefully monitored and quality control tests done to track systematic effects and performance variations over time to maximize the possibility of getting high quality data.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE