Quality Tools and their Application in the Production of Cellular Reagents for Screening Assays.
Poster Nov 13, 2009
Sharon Davies, Lynne Smith, Lisa Bailey, Martyn Birch, Rahman Ismail, Peter Tatnell, Michelle Doyle, Liz Price and J.M Kendall, GE Healthcare
The use of mammalian cell based assays in compound screening continues to grow. Over 50% of all screening assays (HTS and HCA) now utilise cells or cell derived reagents.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE