Quantitative Evaluation of High-Abundance Serum Proteins Depletion
Poster Jan 10, 2007
Stempfer R., Gerner C., Reichel C. and Noehammer C.
Identification of low abundant proteins requires relative enrichment. We have tested systems for specific removal of high abundance proteins. Efficiency of albumin and IgG removal was assessed by ELISA and 2-DE. To estimate unspecific binding, six cytokines were spiked into serum. Recovery rates were determined with a cytometric bead assay.